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- Title
Selective oxidation of UDP-glucose to UDP-glucuronic acid using permeabilized Schizosaccharomyces pombe expressing human UDP-glucose 6-dehydrogenase.
- Authors
Weyler, Christian; Bureik, Matthias; Heinzle, Elmar
- Abstract
Objectives: To use permeabilized cells of the fission yeast, Schizosaccharomyces pombe, that expresses human UDP-glucose 6-dehydrogenase (UGDH, EC 1.1.1.22), for the production of UDP-glucuronic acid from UDP-glucose. Results: In cell extracts no activity was detected. Therefore, cells were permeabilized with 0.3 % (v/v) Triton X-100. After washing away all low molecular weight metabolites, the permeabilized cells were directly used as whole cell biocatalyst. Substrates were 5 mM UDP-glucose and 10 mM NAD. Divalent cations were not added to the reaction medium as they promoted UDP-glucose hydrolysis. With this reaction system 5 mM UDP-glucose were converted into 5 mM UDP-glucuronic acid within 3 h. Conclusions: Recombinant permeabilized cells of S. pombe can be used to synthesize UDP-glucuronic acid with 100 % yield and selectivity.
- Subjects
OXIDATION; UDP-glucuronate decarboxylase; SCHIZOSACCHAROMYCES pombe; UDP-glucose dehydrogenase; GENE expression
- Publication
Biotechnology Letters, 2016, Vol 38, Issue 3, p477
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-015-1995-x