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- Title
Permeabilized probiotic Lactobacillus plantarum as a source of β-galactosidase for the synthesis of prebiotic galactooligosaccharides.
- Authors
Gobinath, Duraiswamy; Prapulla, Siddalingaiya
- Abstract
Permeabilized probiotic Lactobacillus plantarum was used as a source of β-galactosidase for the synthesis of galactooligosaccharides (GOS) from lactose. β-galactosidase activity was highest when galactose (1,724 Miller Units) was used as a carbon source compared to lactose, sucrose or glucose at 37 °C, 18 h. Permeabilized cells had the highest transgalactosylation activity resulting in 34 % (w/w) GOS synthesis from 40 % (w/v) lactose at 50 °C over 12 h. HPLC revealed that the GOS were composed of 13 % disaccharides (non-lactose), 17 % trisaccharides and 4 % tetrasaccharides that were further confirmed by ESI-MS.
- Subjects
LACTOBACILLUS plantarum; PREBIOTICS; PROBIOTICS; LACTOBACILLUS; CHEMICAL synthesis
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 1, p153
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1345-9