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- Title
Cloning and expression of two plant proteins: similar antimicrobial activity of native and recombinant form.
- Authors
Capparelli, R.; Palumbo, D.; Iannaccone, M.; Ventimiglia, I.; Di Salle, E.; Capuano, F.; Salvatore, P.; Amoroso, M. G.
- Abstract
Antimicrobial peptides and proteins are being studied with increasing interest because of their broad range antimicrobial activity. Among plant antimicrobial proteins, the wheat seed polypeptides, puroindoline a and puroindoline b, are particularly interesting because of their established antibacterial activity. In this paper we describe different strategies used to clone His tagged and GST tagged puroindolines obtaining 1.5 mg recombinant protein from 1 l culture. The antimicrobial activity of recombinant and native puroindolines was comparable.
- Subjects
PLANT proteins; WHEAT; RECOMBINANT proteins; ANTI-infective agents; GENETIC engineering; CLONING
- Publication
Biotechnology Letters, 2006, Vol 28, Issue 13, p943
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-006-9031-9