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- Title
Genetics and human conception: DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease.
- Authors
Levinson, Gene; Keyvanfar, Keyvan; Wu, Joy C.; Fugger, Edward F.; Fields, Rita A.; Harton, Gary L.; Palmer, Frances T.; Sisson, Michael E.; Starr, Karin M.; Dennison-Lagos, Lisa; Calvo, Lucrecia; Sherins, Richard J.; Bick, David; Schulman, Joseph D.; Black, Susan H.
- Abstract
We report the world's first clinical pregnancy resulting from DNA-based enrichment for X-bearing human spermatozoa, for prevention of X-linked hydrocephalus. Sperm separation was followed by embryo biopsy and nested multiplex polymerase chain reaction (PCR) for gender determination. Enriched populations of X-bearing spermatozoa ranging from 80 to 89% pure as determined by fluorescence in-situ hybridization (FISH) resulted in in-vitro fertilization (IVF) rates indistinguishable from normal IVF procedures (65%). In two separate biopsy procedures, 7/9 and 15/16 of the resulting embryos were determined to be female by multiplex PCR. Embryo transfer resulted in a karyotypically normal female fetus. This technique should be widely applicable to gender selection for the prevention of genetic disorders.
- Publication
Human Reproduction, 1995, Vol 10, Issue 4, p979
- ISSN
0268-1161
- Publication type
Article