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- Title
Biofilms en instalaciones alimentarias militares. Comparación de técnicas rápidas de vigilancia y verificación.
- Authors
A., Carbajo-Otero; D., Lozano-Benito; F. J., Aranda-Narváez; A., Cereceda-Chacón; A., Ripodas-Navarro; A., Zamora-Benito; D., Fernández-Moreira
- Abstract
Introduction: Biofilm-forming pathogens increase the risk of biological food contamination in food industries, thus it is necessary to quantify and qualify the dangers present in them. Objective: To demonstrate the presence of bacterial biofilms on the work surfaces of military food facilities using different rapid hygienic control techniques. Materials and methods: An amount of 550 samples were analyzed among 10 military food facilities: Each one had 5 sampling points (n = 50) and in each of them 11 samples were obtainded on a surface of 100 cm2 to assess the presence of biofilms and to establish the correlation between different analytical techniques: determination of adenosin triphosphate (ATP) using 3M Clean-Trace ™ NG3 luminometer, protein residue titration with Clean-Trace ™ Surface Protein Plus swabs, mesophilic aerobic microorganism (AC) counts using TEMPO® and Petrifilm ™ system, and visualization of biofilms using enzymatic reaction with Biofinder®. Results: A statistically significant correlation (p <0.05) was found between ATP determinations and the presence of biofilms and protein residues. Linearity with statistical significance (R2 0.422, p <0.05) was also found between ATP and AC count by TEMPO®. Correlation and concordance between AC counts were observed using the TEMPO® system and through Petrifilm ™ (intraclass correlation coefficient 0.463, 95% CI: 0.179-0.673, p <0.05). At no sampling point (SP) were pathogenic bacteria detected. Conclusion: Combining quick techniques should be considered in surveillance procedures of Hazard Analysis and Critical Control Points (HACCP).
- Subjects
BIOFILMS; FOOD contamination; FOOD industry; ADENOSINE triphosphatase; AEROBIC bacteria
- Publication
Sanidad Militar, 2022, Vol 78, Issue 2, p89
- ISSN
1887-8571
- Publication type
Article
- DOI
10.4321/S1887-85712022000200006