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- Title
Desarrollo de un método de detección de Ocratoxina A (OTA) mediante Cromatografía Líquida de Alta Resolución con Detector de Masas en Tiempo de Vuelo.
- Authors
Guamán, Collaguazo C. E.; Peraile, Muñoz I.; Fernández, Martínez C.; Cabria, Ramos J. C.; Gil, García M.
- Abstract
Antecedents: Ochratoxin A (OTA) is a mycotoxin produced by some fungi (Aspergillus y Penicillium). It can be found in several types of food, depending on both production and storage conditions. It has carcinogenic, nephrotoxic, teratogenic, immunotoxic and neurotoxic properties. It is toxic by inhalation and by ingestion. Since this toxin has a high stability and its extraction is relatively simple, it represents a serious public health and safety problem. Therefore, in order to help ensure the safety of all the citizens, it is necessary to develop a fast and sensitive ochratoxin detection method. In this sense, liquid chromatography coupled to a time-of-flight mass detector (HPLC-MSD (TOF)) is revealed as a very suitable diagnostic technique to achieve a rapid and specific detection of this mycotoxin. Aim: Development of a fast and specific method of OTA using HPLCMSD (TOF). Material and Methods: Thirty-six assays were tested with different chromatographic and spectrometric conditions. In all the assayed cases, HPLC 1200 and an MSD (TOF) 6210 with electrospray ionization source were used, (Agilent Technologies). Results: The chromatogram obtained showed the peak of OTA at a retention time of 1,791 ± 0,009 minutes. In its mass spectrum it is observed both the molecular ion [M+H]+ at 404,07986 uma and its characteristic isotopic profile. Conclusions: The OTA detection method optimized by HPLC-MSD (TOF) is fast and specific. This method could be suitable for the detection of other toxins, allowing the creation of a database for toxins suspected of being used as bioterrorist agents (or biological warfare agents).
- Subjects
OCHRATOXINS; HIGH performance liquid chromatography; MYCOTOXINS; CARCINOGENICITY testing; CHROMATOGRAMS
- Publication
Sanidad Militar, 2018, Vol 74, Issue 4, p230
- ISSN
1887-8571
- Publication type
Article
- DOI
10.4321/S1887-85712018000400003