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- Title
The effect of marT gene on biofilm production of Salmonella Typhimurium.
- Authors
YENER İLÇE, Burcu; AKÇELİK, Nefse
- Abstract
This study investigates the effect of MarT regulator protein on the production of Salmonella Typhimurium biofilm under in vitro culture conditions. Te biofilm formation showed a statistically significant decrease compared to the wild-type strain at the two consecutive concentrations of arabinose (0.02% and 0.01%) in which the production of MarT protein was promoted to the highest level. When comparing the biofilm formation capacity to the wild type, the mutants containing a mutation in the genes investigated in terms of marT regulation showed statistically significant reductions, which were identified as fliA (59%), fliC (40%), fimA (46%), fidL (25%), and misL (25%) mutants. Te bioflm structures of all strains used in this study were detected as the rdar morphotype in agar plates. Among the genes examined, fimA, slsA, fliA, dps, cheA, and wzzB genes were found to have a direct effect on the pellicle formation of S. Typhimurium. Finally, the biofilm formation features of the mutant strain, in which marT expression is induced, on glass, stainless steel, and polystyrene were also examined, and it was determined that the ability to produce bioflm decreased on all surfaces.
- Subjects
BIOFILMS; SALMONELLA typhimurium; GENETIC mutation; GENE expression; POLYSTYRENE; COMPARATIVE studies
- Publication
Turkish Journal of Biology, 2015, Vol 39, Issue 5, p722
- ISSN
1300-0152
- Publication type
Article
- DOI
10.3906/biy-1501-25