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- Title
Poly(benzoxazine) as an Immobilization Matrix for Miniaturized ATP and Glucose Biosensors.
- Authors
Ziller, Charlotte; Lin, Jing; Knittel, Peter; Friedrich, Laura; Andronescu, Corina; Pöller, Sascha; Schuhmann, Wolfgang; Kranz, Christine
- Abstract
The reproducible immobilization of enzymes represents a key requirement in developing sensitive and fast-responding amperometric microbiosensors. A microbiosensor for the respective detection of glucose and adenosine-5′-triphosphate (ATP) is presented by using a poly(benzoxazine) derivative for the entrapment of the enzymes glucose oxidase (GOD) and hexokinase (HEX) at platinum (Pt) microelectrodes (MEs). For glucose, a sensitivity of 123.05±10.78 pA/mM ( n=5) was obtained, which shows twice as high sensitivity compared to microbiosensors that use electrophoretic paints as the immobilization matrix for the same size ME (radius: 25 μm). For the determination of ATP, a sensitivity of 48.47±5.12 pA/μM and a signal-to-noise ratio of 40 at physiological pH values were obtained. Apart from their enhanced sensitivity, a significant improvement of these sensors is related to their improved mechanical stability. The applicability of these poly(benzoxine)-based microbiosensors for ATP detection was demonstrated with measurements at receptor protein tyrosine phosphatase zeta (PTPRζ) osteoblastic cells during mechanical stimulation.
- Subjects
PHYSIOLOGICAL effects of glucose; ADENOSINES; BENZOXAZINES; MICROELECTRODES; PROTEIN-tyrosine kinases; ELECTRODE testing
- Publication
ChemElectroChem, 2017, Vol 4, Issue 4, p864
- ISSN
2196-0216
- Publication type
Article
- DOI
10.1002/celc.201600765