We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Association between Gut Microbiota and Uremia of Chronic Kidney Disease.
- Authors
Kim, Ji Eun; Kim, Hyo-Eun; Park, Ji In; Cho, Hyunjeong; Kwak, Min-Jung; Kim, Byung-Yong; Yang, Seung Hee; Lee, Jung Pyo; Kim, Dong Ki; Joo, Kwon Wook; Kim, Yon Su; Kim, Bong-Soo; Lee, Hajeong
- Abstract
Chronic kidney disease (CKD)-associated uremia aggravates—and is aggravated by—gut dysbiosis. However, the correlation between CKD severity and gut microbiota and/or their uremic metabolites is unclear. We enrolled 103 CKD patients with stage 1 to 5 and 46 healthy controls. We analyzed patients' gut microbiota by MiSeq system and measured the serum concentrations of four uremic metabolites (p-cresyl sulfate, indoxyl sulfate, p-cresyl glucuronide, and trimethylamine N-oxide) by liquid chromatography–tandem mass spectrometry. Serum concentrations of the uremic metabolites increased with kidney function deterioration. Gut microbial diversity did not differ among the examined patient and control groups. In moderate or higher stage CKD groups, Oscillibacter showed positive interactions with other microbiota, and the proportions of Oscillibacter were positively correlated with those of the uremic metabolites. The gut microbiota, particularly Oscillibacter, was predicted to contribute to pyruvate metabolism which increased with CKD progression. Relative abundance of Oscillibacter was significantly associated with both serum uremic metabolite levels and kidney function. Predicted functional analysis suggested that kidney-function-associated changes in the contribution of Oscillibacter to pyruvate metabolism in CKD may greatly affect the gut environment according to kidney function, resulting in dysbiosis concomitant with uremic toxin production. The gut microbiota could be associated with uremia progression in CKD. These results may provide basis for further metagenomics analysis of kidney diseases.
- Subjects
GUT microbiome; CHRONIC kidney failure; TANDEM mass spectrometry; LIQUID chromatography-mass spectrometry; UREMIA; KIDNEY diseases
- Publication
Microorganisms, 2020, Vol 8, Issue 6, p907
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms8060907