We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bioglycerol (C3) upgrading to 2,3‐butanediol (C4) by cell‐free extracts of Enterobacter aerogenes NCIM 2695.
- Authors
Parate, Roopa; Borgave, Mrunal; Dharne, Mahesh; Rode, Chandrashekhar
- Abstract
BACKGROUND: Production of biobased chemicals from renewable resources is a green starring approach that serves as a substitute to petroleum derivatives. Bioglycerol, with its growing production as a co‐product of biodiesel, is an attractive low‐cost feedstock for the synthesis of platform chemicals by microbial fermentation. 2,3‐butanediol (2,3‐BDO) is amongst the top biorefinery platform chemicals that can be produced by glycerol fermentation. RESULTS: The 'Circular Economy' concept is demonstrated by converting the by‐product bioglycerol using a cell‐free extract of Enterobacter aerogenes NCIM 2695 (National Collection of Industrial Microorganisms, NCIM), yielding 22 g L−1 2,3‐BDO, in 96 h, 98% atom economy and 0.4 g/g E factor. The cell‐free bioglycerol conversion to 2,3‐BDO was proved using a modified procedure for determining glycerol dehydrogenase enzyme assay by protein analysis and it was also shown to be cell‐bounded. CONCLUSION: Our study offers an effective utilization of the leftover material (i.e. cell‐free extract) that biocatalysed C3 to C4 diol, which adds value to the overall economics of the process using only crude glycerol (C3) itself as a fermentative medium. © 2020 Society of Chemical Industry
- Subjects
SOCIETY of Chemical Industry (Great Britain); ENTEROBACTER aerogenes; BUTANEDIOL; GLYCERIN; VALUE (Economics); PROTEIN analysis; CHEMICAL synthesis
- Publication
Journal of Chemical Technology & Biotechnology, 2021, Vol 96, Issue 5, p1316
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.6650