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- Title
Tubulin Polyglutamylation by TTLL1 and TTLL7 Regulate Glutamate Concentration in the Mice Brain.
- Authors
Ping, Yashuang; Ohata, Kenji; Kikushima, Kenji; Sakamoto, Takumi; Islam, Ariful; Xu, Lili; Zhang, Hengsen; Chen, Bin; Yan, Jing; Eto, Fumihiro; Nakane, Chiho; Takao, Keizo; Miyakawa, Tsuyoshi; Kabashima, Katsuya; Watanabe, Miho; Kahyo, Tomoaki; Yao, Ikuko; Fukuda, Atsuo; Ikegami, Koji; Konishi, Yoshiyuki
- Abstract
As an important neurotransmitter, glutamate acts in over 90% of excitatory synapses in the human brain. Its metabolic pathway is complicated, and the glutamate pool in neurons has not been fully elucidated. Tubulin polyglutamylation in the brain is mainly mediated by two tubulin tyrosine ligase-like (TTLL) proteins, TTLL1 and TTLL7, which have been indicated to be important for neuronal polarity. In this study, we constructed pure lines of Ttll1 and Ttll7 knockout mice. Ttll knockout mice showed several abnormal behaviors. Matrix-assisted laser desorption/ionization (MALDI) Imaging mass spectrometry (IMS) analyses of these brains showed increases in glutamate, suggesting that tubulin polyglutamylation by these TTLLs acts as a pool of glutamate in neurons and modulates some other amino acids related to glutamate.
- Subjects
TUBULINS; GLUTAMIC acid; GLUTAMATE receptors; KNOCKOUT mice; MASS spectrometry; MICE
- Publication
Biomolecules (2218-273X), 2023, Vol 13, Issue 5, p784
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom13050784