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- Title
Identification of IGF-1 Gene Polymorphism Using PCR-RFLP for Improving Goat Meat Evaluation in Carpatina Breed.
- Authors
Lazar, Cristina; Pelmus, Rodica Stefania; Gras, Alexandru Mihail; Rotar, Mircea Catalin; Ghiţă, Elena
- Abstract
The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve goat meat evaluation. Insulin like growth factor -1 play an important stimulator role in skeletal growth, cell differentiation and metabolism. Also it has an important role in control of hair cycles and it is involved in development of wool fiber. Therefore, it is considered as an appropriate candidate gene for meat quality evaluation. Blood samples were collected from 12 Carpatina kids and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of IGF-1 was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. In the present study heterozygous individuals were identified with genotype BC (25%) and homozygous individuals for the genotype BB (75%). B allele frequency was 0.87 and for allele C was 0.13. Observed and expected values of IGF-1 genotypes were found in Hardy Weinberg equilibrium, after χ2 test was calculated. Further investigation is necessary to be carried out on a high number of animals in order to discover other mutations correlated with meat quality in Carpatina goat.
- Subjects
GROWTH factors; POLYMERASE chain reaction; GENETIC polymorphisms
- Publication
Scientific Papers: Animal Science & Biotechnologies / Lucrari Stiintifice: Zootehnie si Biotehnologii, 2018, Vol 51, Issue 1, p97
- ISSN
1841-9364
- Publication type
Article