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- Title
Antiproliferative activity of vanilla stem extract on MCF-7 breast cancer cell lines.
- Authors
Amalnath, A.; Gowri, V.; Punnagai, K.; Harshitha, S.
- Abstract
Background: Cancer is one of the major health problem faced by developing countries. Breast cancer was found to be the second foremost reason of the death worldwide. Many of the existing anticancer drugs have serious side effects. In the current scenario, the use of herbal extract as anticancer drugs is increasing. In India, some orchids were used as medicine, one such an orchid is Vanilla planifolia. Hence, attempt has been made to investigate the in vitro antiproliferative potential of ethanolic extract of stems of V. planifolia (ESVP) against MCF-7 breast cancer cell lines. Materials and Methods: The antiproliferative effect of ethanolic ESVP against MCF breast cancer cell line was estimated by the 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl--tetrazolium bromide assay. Various concentrations of ethanolic ESVP were subjected to the study which includes 7.8 mcg/mL, 15.6 mcg/mL, 31.2 mcg/mL, 62.5 mcg/mL, 125 mcg/mL, 250 mcg/mL, 500 mcg/mL, and 1000 mcg/mL. The IC50 value was calculated. The effect of extract on the cell cycle distribution was investigated using flow cytometry. All experiments were performed in triplicates and statistical analysis was performed using GraphPad Prism 5 version 5.01. Results: The MCF-7 cell viability with various concentrations of ethanolic ESVP 7.8 mcg/mL, 15.6 mcg/mL, 31.2 mcg/mL, 62.5 mcg/mL, 125 mcg/mL, 250 mcg/mL, 500 mcg/mL, and 1000 mcg/mL was found to be 82.17%, 74.285%, 66.99%, 58.71%, 50.43%, 43.67%, 35.52%, and 27.30%. The IC50 value was found to be 125 mcg/ml. The cell viability activity was found to be dose dependent. In vanilla stem extract-treated MCF-7 cells, 16% cells showed apoptosis. It was higher than the control. G0/G1 stage showed 50.72% and S phase showed 12.3% and 17.42% of cells in G2/M phase. Conclusions: There was a significant antiproliferative activity observed in ethanolic ESVP against MCF-7 breast cancer cell lines.
- Subjects
INDIA; CELL lines; CANCER cells; BREAST cancer; VANILLA; CELL cycle
- Publication
Drug Invention Today, 2020, Vol 13, Issue 6, p855
- ISSN
0975-7619
- Publication type
Article