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- Title
Development of a fast and low-cost age determination method in spotted deer, Axis axis.
- Authors
Vipin; SHARMA, Vinita; GUPTA, Sandeep K.; SHARMA, Chandra P.; SANKAR, Kalyanasundaram; GOYAL, Surendra P.
- Abstract
We developed a protocol which determines age in spotted deer, Axis axis (Erxleben, 1777) through cementum layers in the tooth which is economical and requires lesser time (±19 hours). The permanent first incisor teeth from mandibles of known estimated ages (2, 4, 5, 8 and 9 years) were ground longitudinally on median plane on a set of sandpapers with hand to a thickness of around 57 μm, decalcified, stained with hematoxylin, differentiated in acid alcohol and mounted on a glass slide and count cementum layers under microscope at 100 or 200× magnification. Presence of 1, 3, 4, 7 and 8 cementum layers was observed for the tooth of ages of 2, 4, 5, 8 and 9 years, respectively. The final age in the year was calculated by adding one to the counted number of cementum layer. Our results were in compliance with the pre-estimated age of the mandibles. This suggested protocol saves time and money and is easy to perform with minimum requirements. The technique was validated using "blind" duplicate test (n = 2) and the first molar tooth of known estimated age (n = 1). Our suggested protocol has significant relevance for estimating the age of other animals and wildlife forensics.
- Subjects
AXIS axis; CEMENTUM; TEETH; TIGERS; DENTAL maturity
- Publication
Folia Zoologica, 2018, Vol 67, Issue 3/4, p186
- ISSN
0139-7893
- Publication type
Article
- DOI
10.25225/fozo.v67.i3-4.a9.2018