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- Title
Mutation of p53 Gene and Its Correlation with the Clinical Outcome in Dogs with Lymphoma.
- Authors
Koshino, A.; Goto ‐ Koshino, Y.; Setoguchi, A.; Ohno, K.; Tsujimoto, H.
- Abstract
Background p53 plays a key role in the apoptotic event induced by chemotherapeutic agents. Mutation of p53 gene has been observed in various spontaneous tumors in humans and is associated with a poor prognosis. p53 abnormalities have been evaluated in several tumors in dogs; however, the association of p53 gene mutation with clinical outcome in dogs with lymphoma has not been documented. Hypothesis/Objectives The aim of this study was to examine p53 mutation in canine lymphoma cells and its association with the clinical outcome. Animals Forty-three dogs with previously untreated high-grade lymphoma referred to the University of Tokyo were included in this study. Methods Prospective cohort study. We examined p53 gene (exon 4-8) mutation in the tumor tissues from 43 dogs with lymphoma using PCR- SSCP (polymerase chain reaction - single-strand conformational polymorphism) analysis, followed by nucleotide sequencing of the abnormal bands. Results Of the 43 dogs, 7 dogs (16%) had p53 mutation, whereas 36 dogs (84%) were devoid of p53 mutation. Overall response rate after remission induction was significantly lower (33% versus 88%, P = .002) in dogs with lymphomas having p53 mutation than those with lymphomas devoid of p53 mutation. Overall survival time was significantly shorter (67 days versus 264 days, P = .004) in dogs with lymphoma with p53 mutation than those with lymphoma retaining wild-type p53. Conclusion and Clinical Importance Mutations of p53 gene were detected in a proportion of canine lymphoma cells from untreated dogs and can be associated with a poor prognosis.
- Subjects
P53 antioncogene; LYMPHOMAS in dogs; ANTINEOPLASTIC agents; GENETIC mutation; COHORT analysis
- Publication
Journal of Veterinary Internal Medicine, 2016, Vol 30, Issue 1, p223
- ISSN
0891-6640
- Publication type
Article
- DOI
10.1111/jvim.13807