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- Title
In vivo and in vitro anti-natriuretic activity of twigs fraction from Dorstenia picta: A possible mechanism.
- Authors
Florence, Ngueguim Tsofack; Nicolas, Markadieu; Raphael, Crutzen; Hubert, Donfack Jean; Etienne, Dongo; Pierre, Kamtchouing; Theophile, Dimo; Renaud, Beauwens
- Abstract
The present study examine the in vivo effects of Dorstenia Picta (D. picta) on urinary volume and sodium excretion in streptozotocin-induced diabetic rats, and determine a possible mechanism by which the extract increased sodium transport in A6 cells monolayers. Administration of the plant extract at the dose of 150 mg/kg during two weeks decreased urinary volume and sodium excretion. In vitro study showed that, apical application of the plant extract at the dose of 100 μg/mL does not significantly increase sodium transport, whereas basolateral administration provoked a significant (P<0.05) increase of sodium transport in a concentration-dependent manner. The plant extract increases the sodium transport by 69.93% versus 55.41% for insulin and 78.44% for adenosine alter 30 rain. Preincubation of A6 cells monolayers with inhibitor of all adenosine receptors completely suppressed adenosine and plant extract stimulated sodium transport. Interesting is that, the A1 inhibitor receptor (DPCPX), at 100 nM completely abolished the effect of plant extract. The plant extract increased sodium transport by increase PI3-kinase activity and this effect is strongly inhibited by LY-294002. These data also suggest that, the twigs methanol traction from Dorstenia picta increase sodium transport via P13-kinase pathway and requires A1 adenosine receptor.
- Subjects
DORSTENIA; STREPTOZOTOCIN; ADENOSINES; PHOSPHATIDYLINOSITOL 3-kinases; LABORATORY rats; IN vitro studies; PLANT extracts
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2013, Vol 26, Issue 3, p537
- ISSN
1011-601X
- Publication type
Article