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- Title
Oridonin Improves the Sensitivity of Multiple Myeloma Cells to Bortezomib through the PTEN/PI3K/Akt Pathway.
- Authors
Hong Wu; Guihua Zhu; Yizhou Su
- Abstract
Bortezomib is an effective drug for the treatment of multiple myelomas. However, its long-term effectiveness is limited by the development of drug resistance. Oridonin, a natural diterpenoid purified from Rabdosia rubescens, has the potential to diminish resistance to bortezomib. To examine the mechanism underlying the diminution of bortezomib-resistance in multiple myeloma by oridonin, we have created a bortezomib-resistant cell line of multiple myeloma cells (RPMI-8226R). Using MTT assay, apoptosis assays and western blot analysis, we evaluated and compared the effects of oridonin on the cell viability, proliferation, apoptosis, and protein expression levels in bortezomib-resistant (RPMI-8226R) and bortezomib-sensitive (RPMI-8226) myeloma cells. The results show that oridonin sensitized multiple myeloma cells leading to increased apoptosis rate via regulating the PTEN/PI3K/AKT pathway. Furthermore, oridonin activated the expression of PTEN, which is a negative regulator of the PI3K/AKT pathway, while inhibitinged the expression of p-Akt. These results demonstrated that oridonin might be a useful natural compound in bortezomib-resistant multiple myeloma treatment.
- Subjects
APOPTOSIS; CELL differentiation; CELL lines; CELLULAR signal transduction; FLOW cytometry; GENE expression; MULTIPLE myeloma; TERPENES; WESTERN immunoblotting; CELL survival; BORTEZOMIB
- Publication
Current Topics in Nutraceutical Research, 2020, Vol 18, Issue 3, p292
- ISSN
1540-7535
- Publication type
Article
- DOI
10.37290/ctnr2641-452X.18:292-296