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- Title
Investigation of colloidal graphite as a matrix for matrix-assisted laser desorption/ionisation mass spectrometry of low molecular weight analytes.
- Authors
Warren, Alexander D.; Conway, Ulric; Arthur, Christopher J.; Gates, Paul J.
- Abstract
The analysis of low molecular weight compounds by matrix-assisted laser desorption/ionisation mass spectrometry is problematic due to the interference and suppression of analyte ionisation by thematrices typically employed - which are themselves lowmolecular weight compounds. The application of colloidal graphite is demonstrated here as an easy to use matrix that can promote the ionisation of a wide range of analytes including low molecular weight organic compounds, complex natural products and inorganic complexes. Analyte ionisation with colloidal graphite is compared with traditional organicmatrices along with various other sources of graphite (e.g. graphite rods and charcoal pencils). Factors such as ease of application, spectra reproducibility, spot longevity, spot to- spot reproducibility and spot homogeneity (through single spot imaging) are explored. For some analytes, considerable matrix suppression effects are observed resulting in spectra completely devoid of matrix ions. We also report the observation of radical molecular ions [M-•] in the negative ion mode, particularly with some aromatic analytes.
- Subjects
GRAPHITE; DIMERIC ions; SPECTRUM analysis; NUCLEAR spectroscopy; MOLECULAR weights
- Publication
Journal of Mass Spectrometry, 2016, Vol 51, Issue 7, p491
- ISSN
1076-5174
- Publication type
Article
- DOI
10.1002/jms.3774