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- Title
Characterization of vps33<sup>+</sup>, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe.
- Authors
Iwaki, Tomoko; Osawa, Fumi; Onishi, Masayuki; Koga, Takako; Fujita, Yasuko; Hosomi, Akira; Tanaka, Naotaka; Fukui, Yasuhisa; Takegawa, Kaoru
- Abstract
From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33+ gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33+ gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells. Copyright © 2003 John Wiley & Sons, Ltd.
- Publication
Yeast, 2003, Vol 20, Issue 10, p845
- ISSN
0749-503X
- Publication type
Article
- DOI
10.1002/yea.1011