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- Title
PGE<sub>2</sub> promotes macrophage recruitment and neovascularization in murine wet-type AMD models.
- Authors
Zhan, Pengfei; Cui, Yuqing; Cao, Yujuan; Bao, Xun; Wu, Meili; Yang, Qian; Yang, Jiahui; Zheng, Haohan; Zou, Jian; Xie, Tianhua; Cai, Jiping; Yao, Yong; Wang, Xiaolu
- Abstract
Age-related macular degeneration (AMD), a progressive chronic disease of the central retina, is a leading cause of blindness worldwide. Activated macrophages recruited to the injured eyes greatly contribute to the pathogenesis of choroidal neovascularization (CNV) in exudative AMD (wet AMD). This study describes the effects of cyclooxygenase-2 (COX2)/prostaglandin E2 (PGE2) signalling on the macrophage activation and CNV formation of wet AMD. In a mouse model of laser-induced wet AMD, the mice received an intravitreal injection of celecoxib (a selective COX2 inhibitor). Optical coherence tomography (OCT), fundus fluorescein angiography (FFA), choroidal histology of the CNV lesions, and biochemical markers were assessed. The level of PGE2 expression was high in the laser-induced CNV lesions. Macrophage recruitment and CNV development were significantly less after celecoxib treatment. E-prostanoid1 receptor (EP1R)/protein kinase C (PKC) signalling was involved in M2 macrophage activation and interleukin-10 (IL-10) production of bone marrow-derived macrophages (BMDMs) in vitro. In addition, IL-10 was found to induce the proliferation and migration of human choroidal microvascular endothelial cells (HCECs). Thus, the PGE2/EP1R signalling network serves as a potential therapeutic target for CNV of the wet-type AMD. AXv86vuL_n_YsWmKFp4z_2 Video abstract
- Subjects
MACULAR degeneration; NEOVASCULARIZATION; MACROPHAGES; MACROPHAGE activation; OPTICAL coherence tomography; RETROLENTAL fibroplasia; PROSTAGLANDIN receptors
- Publication
Cell Communication & Signaling, 2022, Vol 20, Issue 1, p1
- ISSN
1478-811X
- Publication type
Article
- DOI
10.1186/s12964-022-00973-6