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- Title
Kinetics of organic solvent-soluble and native lipase.
- Authors
Tsuzuki, Wakako; Akasaka, Kazuaki; Kobayashi, Shoichi; Suzuki, Tateo
- Abstract
Organic solvent-soluble lipase was prepared with a synthetic detergent. The solvent-soluble lipase was presumed to be a complex of the enzyme and the detergent. To investigate the effect of the detergent attachment to the enzyme on the reaction properties, the kinetics of the solvent-soluble lipase and of the native lipase were estimated by using glycerides in a homogeneous solution of buffer and tetrahydrofuran. Analysis of the direct interaction between the enzyme and the substrate could serve to characterize the steric structure around the active site of the lipase. The K values also differentiated the solvent-soluble lipase from the native lipase. These findings show for the first time that the detergent surrounding the enzyme molecule may affect not only the solubility of the enzyme, but its kinetics as well.
- Publication
Journal of the American Oil Chemists' Society (JAOCS), 1995, Vol 72, Issue 11, p1333
- ISSN
0003-021X
- Publication type
Article
- DOI
10.1007/BF02546208