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- Title
Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene.
- Authors
Xiao-Jun Ji; He Huang; Jian-Guo Zhu; Lu-Jing Ren; Zhi-Kui Nie; Jun Du; Shuang Li
- Abstract
Ethanol was a major byproduct of 2,3-butanediol (2,3-BD) fermentation by Klebsiella oxytoca ME-UD-3. In order to achieve a high efficiency of 2,3-BD production, K. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the aldA gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. The carbon flux to 2,3-BD was enhanced by eliminating the byproducing ethanol and at the same time reducing the accumulation of another byproduct acetoin. At last, by fed-batch culturing of the mutant, the final 2,3-BD titer up to 130 g/l with the productivity of 1.63 g/l.h and the 2,3-BD yield relative to glucose of 0.48 g/g was obtained.
- Subjects
ALCOHOL; KLEBSIELLA; ALDEHYDE dehydrogenase; DEHYDROGENASES; ACETALDEHYDE; GENES; TETRACYCLINES; BIOCHEMICAL engineering; ALDEHYDES
- Publication
Applied Microbiology & Biotechnology, 2010, Vol 85, Issue 6, p1751
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-009-2222-2