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- Title
Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccumL.).
- Authors
Minmin Sun; Yueming Yan; Yi Jiang; Yinghua Xiao; Yingkao Hu; Minhua Cai; Yaxuan Li; Hsam, Sai L. K.; Zeller, Friedrich J.
- Abstract
Cultivated emmer (Triticum dicoccum, 2n=4x=28,AABB) is closely related to bread wheat and possesses extensive allelic variations in high molecular weight glutenin subunit (HMW-GS) composition. These alleles may be an important genetic resource for wheat quality improvement. To isolate and clone HMW-GS genes from cultivated emmer, two pairs of allele-specific (AS) PCR primers were designed to amplify the coding sequence of y-type HMW-GS genes and their upstream sequences, respectively. The results showed that single bands of strong amplification were obtained through AS-PCR of genomic DNA from emmer. After cloning and sequencing the complete sequence of coding and 5′-flanking regions of a y-type subunit gene atGlu-A1locus was obtained. Nucleotide and deduced amino acid sequences analysis showed that this gene possessed a similar structure as the previously reportedAygene from common wheat, and is hence designated asAy1 d. The distinct feature of theAy1 d gene is that its coding region contains four stop codons and its upstream region has a 85-bp deletion in the same position of theAygene, which are probably responsible for the silencing of y-type subunit genes atGlu-A1locus. Phylogenetic analysis of HMW glutenin subunit genes from differentTriticumspecies and genomes were also carried out.
- Subjects
CLONING; EMMER wheat; GENES; GENETIC engineering; GENETICS
- Publication
Hereditas, 2004, Vol 141, Issue 1, p46
- ISSN
0018-0661
- Publication type
Article
- DOI
10.1111/j.1601-5223.2004.01835.x