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- Title
Apoptosis of A549 cells by small interfering RNA targeting survivin delivery using poly- β-amino ester/guanidinylated O-carboxymethyl chitosan nanoparticles.
- Authors
Yue Tang; Yun Liu; Yuwen Xie; Jing Chen; Yushun Dou
- Abstract
Gene-based therapeutics has emerged as a promising approach for human cancer therapy. Among a variety of non-viral vectors, polymer vectors are particularly attractive due to their safety and multivalent groups on their surface. This study focuses on guanidinylated O-carboxymethyl chitosan (GOCMCS) along with poly- β-amino ester(PBAE) for siRNA delivery. Binding efficiency of PBAE/siRNA/GOCMCS nanoparticles were characterized by gel electrophoresis. The siRNA-loaded nanoparticles were found to be stable in the presence of RNase A, serum and BALF respectively. Fine particle fraction (FPF) which was determined by a two-stage impinger (TSI) was 57.8% ±2.6%. The particle size and zeta potential of the nanoparticles were 153.8 ±12.54 nm and + 12.2 ±4.94 mV. In vitro cell transfection studies were carried out with A549 cells. The cellular uptake was significantly increased. When the cells were incubated with siSurvivin-loaded nanoparticles, it could induce 26.83% ±0.59% apoptosis of A549 cells and the gene silencing level of survivin expression in A549 cells were 30.93% ±2.27%. The results suggested that PBAE/GOCMCS nanoparticle was a very promising gene delivery carrier.
- Subjects
SMALL interfering RNA; RIBONUCLEASE A; NANOPARTICLES; ESTERS; PARTICULATE matter
- Publication
Asian Journal of Pharmaceutical Sciences, 2020, Vol 15, Issue 1, p121
- ISSN
1818-0876
- Publication type
Article
- DOI
10.1016/j.ajps.2018.09.009