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- Title
Comparison of the Efficiency of Hyperspectral and Pulse Amplitude Modulation Imaging Methods in Pre-Symptomatic Virus Detection in Tobacco Plants.
- Authors
Grishina, Alyona; Sherstneva, Oksana; Zhavoronkova, Anna; Ageyeva, Maria; Zdobnova, Tatiana; Lysov, Maxim; Brilkina, Anna; Vodeneev, Vladimir
- Abstract
Early detection of pathogens can significantly reduce yield losses and improve the quality of agricultural products. This study compares the efficiency of hyperspectral (HS) imaging and pulse amplitude modulation (PAM) fluorometry to detect pathogens in plants. Reflectance spectra, normalized indices, and fluorescence parameters were studied in healthy and infected areas of leaves. Potato virus X with GFP fluorescent protein was used to assess the spread of infection throughout the plant. The study found that infection increased the reflectance of leaves in certain wavelength ranges. Analysis of the normalized reflectance indices (NRIs) revealed indices that were sensitive and insensitive to infection. NRI700/850 was optimal for virus detection; significant differences were detected on the 4th day after the virus arrived in the leaf. Maximum (Fv/Fm) and effective quantum yields of photosystem II (ΦPSII) and non-photochemical fluorescence quenching (NPQ) were almost unchanged at the early stage of infection. ΦPSII and NPQ in the transition state (a short time after actinic light was switched on) showed high sensitivity to infection. The higher sensitivity of PAM compared to HS imaging may be due to the possibility of assessing the physiological changes earlier than changes in leaf structure.
- Subjects
PULSE amplitude modulation; POTATO virus X; GREEN fluorescent protein; FLUORESCENCE quenching; PHYTOPATHOGENIC microorganisms
- Publication
Plants (2223-7747), 2023, Vol 12, Issue 22, p3831
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants12223831