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- Title
Metabolic properties of Pachysolen tannophilus mutants producing xylitol and ethanol from D-xylose.
- Authors
Bolotnikova, O.; Meshcheryakova, O.; Mikhailova, N.; Ginak, A.
- Abstract
Activity of the major enzymes of D-xylose metabolism in the mutants of xylose-utilizing yeasts Pachysolen tannophilus selectively producing xylitol or ethanol was studied. The xylitol-producing strain exhibited low activities of xylitol dehydrogenase, xylose reductase with preferential affinity to NADPH, NAD-dependent malate dehydrogenase, and cytochrome c oxidase (4.40, 4.80, 1.87, and 0.28 μmol mg min, respectively). The cells of the ethanol-producing mutants exhibited elevated activity of NADH/NADPH-xylose reductase, xylitol dehydrogenase, 1-glycerophosphate dehydrogenase, and lactate dehydrogenase to 6.80, 8.60, 4.68, and 16.48 μmol mg min, respectively. Effect of the NADPH/NADH imbalance on ethanol production accumulation and xylitol accumulation is discussed.
- Subjects
XYLOSE; XYLITOL; DEHYDROGENASE genetics; DEHYDROGENASES regulation; ENZYME regulation
- Publication
Microbiology (00262617), 2015, Vol 84, Issue 4, p485
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1134/S0026261715040050