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- Title
Cellular responses to Schistosoma japonicum cathepsin D aspartic protease.
- Authors
Verity, Christiana K.; Mcmanus, Donald P.; Brindley, Paul J.
- Abstract
Summary Lymphocyte proliferation and cytokine production were measured in groups of mice vaccinated (but not subsequently challenge infected) with recombinant forms of Schistosoma japonicum cathepsin D aspartic protease, rSjASP1 (expressed in bacteria; enzymatically inactive) and rSjASP2 (expressed in insect cells; enzymatically active). Both forms of the schistosome enzyme induced significant proliferation of splenocytes recovered from vaccinated mice, and expression of interferon (IFN)-γ, interleukin (IL)-4 and IL-10 mRNA in these cells was detected using reverse transcriptase-polymerase chain reaction. Secretion of IFN-γ, IL-4 and IL-10 by splenocytes from vaccinated mice was confirmed and quantified using enzyme-linked immunosorbent assay. IFN-γ was the most abundant cytokine produced, followed by IL-4 and IL-10 in rank order. These findings indicated that vaccination of mice with the schistosome protease induces a mixed Th1/Th2 cytokine response, which may explain the modest level of protection after challenge infection in cathepsin d-vaccinated mice, reported previously.
- Subjects
SCHISTOSOMA japonicum; PROTEOLYTIC enzymes; CYTOKINES
- Publication
Parasite Immunology, 2002, Vol 24, Issue 7, p363
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1046/j.1365-3024.2002.00475.x