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- Title
Development and Characterization of a Novel Live Attenuated Vaccine Against Enteric Septicemia of Catfish.
- Authors
Abdelhamed, Hossam; Lawrence, Mark L.; Karsi, Attila
- Abstract
Edwardsiella ictaluri is a Gram-negative intracellular pathogen causing enteric septicemia of channel catfish (ESC). Type six secretion system (T6SS) is a sophisticated nanomachine that delivers effector proteins into eukaryotic host cells as well as other bacteria. In the current work, we in-frame deleted the E. ictaluri evpB gene located in the T6SS operon by allelic exchange. The safety and efficacy of Ei Δ evpB as well as Aquavac-ESC, a commercial vaccine manufactured by Intervet/Merck Animal Health, were evaluated in channel catfish (Ictalurus punctatus) fingerlings and fry by immersion exposure. Our results showed that the Ei Δ evpB strain was avirulent and fully protective in catfish fingerlings. The Ei Δ evpB strain was also safe in catfish fry, and immersion vaccination with Ei Δ evpB at doses 106 and 107 CFU/ml in water resulted in 34.24 and 80.34% survival after wild-type immersion challenge compared to sham-vaccinated fry (1.79% survival). Catfish fry vaccinated with Ei Δ evpB at doses 106, 107, and 108 CFU/ml in water exhibited dose-dependent protection. When compared with Aquavac-ESC, Ei Δ evpB provided significantly higher protection in catfish fingerlings and fry (p < 0.05). Results indicate that the Ei Δ evpB strain is safe and can be used to protect catfish fingerlings and fry against E. ictaluri.
- Subjects
EDWARDSIELLA; SEPSIS; CATFISHES
- Publication
Frontiers in Microbiology, 2018, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2018.01819