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- Title
Regenerative capacity of mesenchymal stem cells in peripheral nerve injury crush model.
- Authors
Üstün, Ramazan; Oğuz, Elif Kaval; Akkoçs, Tunç; Şeker, Ayşe; Türker, Rabia Sena; Keskin, Sıddık
- Abstract
Objective: Current study aimed to elucidate the regenerative capacity of mesenchymal stem cells (MSC) in a peripheral nerve crush model. Methods: Study design was composed of control (n=7), crush (n=7), crush + MSC (treatment) (n=7) groups. Sciatic nerve crush injury model was established in adult male mice. Bone marrow-derived MSC (800,000 cells) was administered to the treatment group with a single intraperitoneal dose. The study was continued for eight weeks. Degeneration and regeneration findings were obtained by functional tests and immunohistochemical techniques. Sensory function test (hotplate/pain nociception latency) and motor function test (sciatic function index) were used for functional evaluation. Immunofluorescence imaging of nerve and target muscle tissue was performed for immunohistochemical evaluation. Axonal markers (neurofilament 200 (NFH), pIH tubulin), myelin markers (MBP, MPÛ) and nerve-muscle junction markers (neurofilament 200, a-bungarotoxin (a-BTX)) were used. Immunoreactivities of proteins were analyzed with ImageJ software. Results: Functional tests performed one week after crush injury showed that the injured groups suffered significant sensory and motor function loss compared to the control group (p<0.05). The tests repeated every week throughout the study showed that the MSC treatment group's functional activity was higher than the crush group (p<0.05), and there was no difference between the control group and the MSC group. In immunohistochemical comparison, immunoreactivities of axonal structures (PHI tubulin, NFH) were significantly higher in control and MSC groups than the crush group (pcO.05); there was no difference between the control group and MSC group. Immunreactivities of myelin proteins (MBP, PÖ) were higher in the control group than the other groups (p<0.002); there was no difference between the crush group and the MSC group. In the immunohistochemical comparison of target muscle tissue, NFH immunoreactivity showed a significant difference; it was the highest in the control group, the MSC group was moderate, and the crush group was the lowest (p<0.002). There was no difference between the groups in the immunoreactivity of a-BNG. Conclusion: MSC treatment carried out the regeneration of the axonal structures of the sciatic nerve. There were signs of healings in myelin and neuromuscular junctions but were not statistically significant. In new studies for the recovery of myelin and target muscle group, the second MSC treatment dose should be tried, and the study should be planned for a more extended period.
- Subjects
MESENCHYMAL stem cells; SCHWANN cells; CRUSH syndrome; NEURONS; PERIPHERAL nervous system; TUBULINS; REGENERATION (Biology); SCIATIC nerve injuries
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2020, Vol 14, pS118
- ISSN
1307-8798
- Publication type
Article