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- Title
Investigation of neuron protective and anticancer effects of pyrogallol: in vitro experiment.
- Authors
Çiçek, Betül; Taghizadehghalehjoughi, Ali; Şentürk, Esra; Şentürk, Murat; Hacımüftüoğlu, Ahmet; Gül, Mustafa
- Abstract
Objective: Neuroblastoma is the most common solid tumor of the childhood and responsible for approximately %15 of cancer related deaths in children. Oxidative stress plays an important role in the pathogenesis of cancer by affecting different stages of cancer formation. Phenolic compounds with antioxidant properties are important to develop new therapeutic strategies for cancer. The aim of this study was to investigate the effects of pyrogallol on neuroblastoma (SH-SY5Y) cell line and primary cortical neuron cells. Methods: SH-SY5Y neuroblastoma and primary cortical neuron cells were grown in the appropriate cell culture mediums. Cells were exposed to pyrogallol at different concentrations (20, 40, 80, 200 pM) for 24 hours. The cell viability was determined by using MTT method and GSH levels were assessed to be the indicator of oxidative effect. The statistical analysis was done by one-way analysis of variance (ANOVA) and Tukey's HSD test. Results: According to MTT analysis results, 200 pM pyrogallol group in cortical neuron culture was found to have reduced cell viability compared to control group (p<0.05), however it was determined that the decreases in 20, 40 and 80 pyrogallol groups were not statistically significant compared to the control group. In SH-SY5Y cell line, pyrogallol (20-200 pM) significantly decreased cell viability compared to the control group (p<0.001). GSH levels showed compatibility with MTT results in both neuron cells and cancer cell line. Conclusion: It has been determined that pyrogallol, which has antioxidant chracteristics, has protective effect on primary neurons, but causes inhibition in SH-SY5Y cancer cell line. However, further studies are needed to clarify the anticancer and neuron protective effects of pyrogallol.
- Subjects
TUKEY'S test; ONE-way analysis of variance; NEURONS; CARCINOGENESIS; CANCER cells
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS60
- ISSN
1307-8798
- Publication type
Article