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- Title
Evaluating the Expression of Oct-4, NANOG, Sox2 and Nucleostemin in Colon Cancer Cell Lines (Caco-2 and HT-29).
- Authors
Amini, Sabrieh; Fathi, Fardin; Parivar, Kazem; Kuchesfahani, Homa Mohseni; Rezaie, Mohammad Jafar; Nikkhoo, Bahram
- Abstract
Objective: Evaluating the expression of Oct-4, NANOG, Sox2 and Nucleostemin in colon cancer cell lines (Caco-2 and HT-29). Materials and Methods: Caco-2 and HT-29 human colon cancer cell lines were cultured in Dulbecco's modified eagles medium (DMEM) and Roswell Park Memorial Institute medium (RPMI) respectively, containing 10% fetal bovin serum (FBS) with 1% peniciline and streptomycinen in 37°, 5% CO2 incubator. Total RNA was isolated using the ISOGEN method. RNA integrity was checked with the use of agarose gel electrophoresis and spectrophotometry. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to examin the samples. The expression of Oct-4 and Nucleostemin at the protein level was further determined using immunocytochemistry. Results: RT-PCR analysis of Caco2 and HT-29 colon cancer cell lines showed expression of Oct-4, NANOG, Sox2 and Nucleostemin genes . Also immunocytochemical analysis confirmed the cytoplasmic and nuclear expression of the Oct-4 protein and Nucleostemin proteins. Conclusion: Collectively, our data confirmed the expression of Oct-4, NANOG, Sox2 and Nucleostemin in colon cancer cells and suggested that their expression can be used as potential tumor markers in diagnosis and br prognosis of colon tumors. These results confirm the potential value of the cancer stem-cell theory in cancer therapy.
- Subjects
CYTOLOGICAL research; COLON cancer; CELL lines; POLYMERASE chain reaction; BIOMARKERS; CANCER treatment
- Publication
Yakhteh Medical Journal, 2010, Vol 12, Issue 2, p223
- ISSN
1561-4921
- Publication type
Article