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- Title
Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells.
- Authors
Liu, Haikun; Guo, Dongsheng; Ruzi, Aynisahan; Chen, Yan; Pan, Tingcai; Yang, Fan; Li, Jialiang; Xu, Kecheng; Zhou, Tiancheng; Qin, Dajiang; Li, Yin-xiong
- Abstract
Human induced pluripotent stem cells (hiPSCs) may provide potential resource for regenerative medicine research, including generation of insulin-producing cells for diabetes research and insulin production. Testosterone (T) is an androgen hormone which promotes protein synthesis and improves the management of type 2 diabetes in clinical studies. Concurrently, co-existed hyperandrogenism and hyperinsulinism is frequently observed in polycystic ovary syndrome, congenital adrenal hyperplasia and some of Wermer's syndrome. However, the relationship among androgens, insulin and the differentiation of pancreatic β cells is still not fully clear. Here we find that T improves the differentiation efficiency of insulin-producing cells from hiPSCs. The addition of T into routine differentiation formula for pancreatic β cells increases the differentiation efficiency from 12% to 35%. The administration of T promotes the expression of key genes associated with β cells differentiation including NGN3, NEUROD1 and INS. This finding benefits the ongoing process to optimize the differentiation protocol of pancreatic β cells from hiPSCs, and provides some degree of understanding the clinical management of T for type 2 diabetes.
- Subjects
INDUCED pluripotent stem cells; TESTOSTERONE; ANDROGENS; INSULIN; PANCREATIC beta cells; CELL differentiation; TYPE 2 diabetes treatment; THERAPEUTICS
- Publication
PLoS ONE, 2017, Vol 12, Issue 6, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0179353