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- Title
A robust system for production of minicircle DNA vectors.
- Authors
Kay, Mark A.; Cheng-Yi He; Zhi-Ying Chen
- Abstract
Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.
- Subjects
DNA; TRANSGENE expression; ESCHERICHIA coli; PLASMIDS; BIOTECHNOLOGY
- Publication
Nature Biotechnology, 2010, Vol 28, Issue 12, p1287
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.1708