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- Title
CLASSICAL AND MODERN TECHNIQUES TO ASSESS THE EFFECTS OF CROP ROTATION ON THE SOIL MICROBIAL COMMUNITY.
- Authors
Varlese, R.; Crispo, D. G.; Ruocco, M.; Vinale, F.; Lanzuise, S.; Nigro, M.; Eid, A. M. E.; Pascale, A.; Manganiello, G.; Matteoli, V.; Stellitano, D.; Marra, R.; Lorito, M.; Woo, S. L.
- Abstract
Microbe-plant interactions in the soil have a large impact on agriculture. Beneficial effects include disease control, plant growth promotion, induced resistance, increased nutrient availability. The objectives of this study were to evaluate the highly complex microbial diversity in soils where legume and wheat crop rotations have been applied, in order to understand the biology and the mechanisms involved in this agricultural system. Samples tested included soils from pea-wheat rotation, no crop, legume only, cereal only. Different approaches using classical and modern molecular techniques were compared. Soil was plated to determine the number of colony forming units (CFUs), the abundance and morphology of the "culturable" microorganisms on selective substrates. Total DNA was extracted for use in molecular techniques of PCR-DGGE and metagenomic analysis; two methods increasingly applied in the field of soil microbiology. Preliminary screening of PCR primers was conducted, and those producing satisfactory results were synthesized with a GC-Clamp for DGGE analysis. The microbial DNA complex was sequenced for metagenomic analysis and functional studies including: identification of genes, metabolic pathways, as well as gene annotation and species identification and abundance. Similarities were noted in from the comparison of traditional and modern methods. Both approaches were able to distinguish microbes from the various crop rotation systems, but only the molecular methods provided the possibility to analyze the non-culturable microflora. Soil microbial diversity has important implications for sustainable agricultural production and management.
- Subjects
CROP rotation; CROPPING systems; PLANT-microbe relationships; PLANTS; MICROBIOLOGY; SOILS; DISEASE resistance of plants
- Publication
Journal of Plant Pathology, 2012, Vol 94, Issue 4, pS4.83
- ISSN
1125-4653
- Publication type
Article