We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Copy number and haplotype variation at the VRN- A1 and central FR- A2 loci are associated with frost tolerance in hexaploid wheat.
- Authors
Zhu, Jie; Pearce, Stephen; Burke, Adrienne; See, Deven; Skinner, Daniel; Dubcovsky, Jorge; Garland-Campbell, Kimberly
- Abstract
Key message: The interaction between VRN - A1 and FR - A2 largely affect the frost tolerance of hexaploid wheat. Abstract: Frost tolerance is critical for wheat survival during cold winters. Natural variation for this trait is mainly associated with allelic differences at the VERNALIZATION 1 ( VRN1) and FROST RESISTANCE 2 ( FR2) loci. VRN1 regulates the transition between vegetative and reproductive stages and FR2, a locus including several tandemly duplicated C- REPEAT BINDING FACTOR ( CBF) transcription factors, regulates the expression of Cold- regulated genes. We identified sequence and copy number variation at these two loci among winter and spring wheat varieties and characterized their association with frost tolerance. We identified two FR- A2 haplotypes-'FR-A2-S' and 'FR-A2-T'-distinguished by two insertion/deletions and ten single nucleotide polymorphisms within the CBF- A12 and CBF- A15 genes. Increased copy number of CBF- A14 was frequently associated with the FR- A2- T haplotype and with higher CBF14 transcript levels in response to cold. Factorial ANOVAs revealed significant interactions between VRN1 and FR- A2 for frost tolerance in both winter and spring panels suggesting a crosstalk between vernalization and cold acclimation pathways. The model including these two loci and their interaction explained 32.0 and 20.7 % of the variation in frost tolerance in the winter and spring panels, respectively. The interaction was validated in a winter wheat F population segregating for both genes. Increased VRN- A1 copy number was associated with improved frost tolerance among varieties carrying the FR- A2- T allele but not among those carrying the FR- A2- S allele. These results suggest that selection of varieties carrying the FR- A2- T allele and three copies of the recessive vrn- A1 allele would be a good strategy to improve frost tolerance in wheat.
- Subjects
WHEAT; PLANT haplotypes; PLANT variation; GENETIC regulation in plants; PLANT proteins; SINGLE nucleotide polymorphisms
- Publication
Theoretical & Applied Genetics, 2014, Vol 127, Issue 5, p1183
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-014-2290-2