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- Title
SK<sub>Ca</sub> Channels Mediate the Medium But Not the Slow Calcium-Activated Afterhyperpolarization in Cortical Neurons.
- Authors
Villalobos, Claudio; Shakkottai, Vikram G.; Chandy, K. George; Michelhaugh, Sharon K.; Andrade, Rodrigo
- Abstract
Pyramidal cells of layer V exhibit robust AHP currents composed of two kinetically and pharmacologically distinguishable currents known as the medium AHP current (ImAHP) and the slow AHP current (IsAHP). ImAHP is blocked by the SKCa channel blockers apamin and bicuculline, whereas IsAHP is resistant to these agents but is inhibited by activation of muscarinic receptors. To test for a role for SKCa channels, we overexpressed KCa2.1 (SKI) and KCa2.2 (SK2), the predominant SKCa subunits expressed in the cortex, in pyramidal cells of cultured brain slices. Overexpression of KCa2.1 and KCa2.2 resulted in a fourfold to fivefold increase in the amplitude of ImAHP but had no detectable effect on IsAHP. As an additional test, we examined IsAHP in a transgenic mouse expressing a truncated SKCa subunit (SK3-1B) capable of acting as a dominant negative for the entire family of SKCa-IKCa channels. Expression of SK3-1B profoundly inhibited ImAHP but again had no discernable effect on IsAHP. These results are inconsistent with the proposal that SKCa channels mediate IsAHP in pyramidal cells and indicate that a different potassium channel mediates this current.
- Subjects
KUPFFER cells; CELLS; EFFERENT pathways; TRANSGENIC mice; TRANSGENIC animals
- Publication
Journal of Neuroscience, 2004, Vol 24, Issue 14, p3537
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.0380-04.2004