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- Title
Evaluation of a new fluorescence quantitative PCR test for diagnosing Helicobacter pylori infection in children.
- Authors
Ou, Zhiying; Xiong, Liya; Li, Ding-You; Geng, Lanlan; Li, Lixia; Chen, Peiyu; Yang, Min; Zeng, Yongmei; Zhou, Zhenwen; Xia, Huimin; Gong, Sitang
- Abstract
<bold>Background: </bold>Numerous diagnostic tests are available to detect Helicobactor pylori (H. pylori). There has been no single test available to detect H. pylori infection reliably. We evaluated the accuracy of a new fluorescence quantitative PCR (fqPCR) for H. pylori detection in children.<bold>Methods: </bold>Gastric biopsy specimens from 138 children with gastritis were sent for routine histology exam, rapid urease test (RUT) and fqPCR. 13C-urea breath test (13C-UBT) was carried out prior to endoscopic procedure. Gastric fluids and dental plaques were also collected for fqPCR analysis.<bold>Results: </bold>38 children (27.5%) were considered positive for H. pylori infection by gold standard (concordant positive results on 2 or more tests). The remaining 100 children (72.5%) were considered negative for H. pylori. Gastric mucosa fqPCR not only detected all 38 H. pylori positive patients but also detected 8 (8%) of the 100 gold standard-negative children or 11 (10.7%) of the 103 routine histology-negative samples. Therefore, gastric mucosa fqPCR identified 46 children (33.3%) with H. pylori infection, significantly higher than gold standard or routine histology (P<0.01). Both gastric fluid and dental plaque fqPCR only detected 32 (23.2%) and 30 (21.7%) children with H. pylori infection respectively and was significantly less sensitive than mucosa fqPCR (P<0.05) but was as sensitive as non-invasive UBT.<bold>Conclusions: </bold>Gastric mucosa fqPCR was more sensitive than routine histology, RUT, 13C-UBT alone or in combination to detect H. pylori infection in children with chronic gastritis. Either gastric fluid or dental plaque PCR is as reliable as 13C-UBT for H. pylori detection.
- Publication
BMC Gastroenterology, 2013, Vol 13, Issue 1, p7
- ISSN
1471-230X
- Publication type
journal article
- DOI
10.1186/1471-230X-13-7