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- Title
Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, in vitro and in vivo.
- Authors
Di Na; Zhi-Dong Lv; Fu-Nan Liu; Yan Xu; Cheng-Gang Jiang; Zhe Sun; Zhi-Feng Miao; Feng Li; Hui-Mian Xu
- Abstract
Background: In this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells in vitro and in vivo. Methods: HMrSV5, a human peritoneal mesothelial cell line, was incubated with supernatants from gastric cancer cells. Morphological changes of HMrSV5 cells were observed. Apoptosis of HMrSV5 cells was observed under a transmission electron microscope and quantitatively determined by MTT assay and flow cytometry. Expressions of apoptosis-related proteins (caspase-3, caspase-8, Bax, bcl-2) were immunochemically evaluated. Results: Conspicuous morphological changes indicating apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. In vivo, peritoneal tissues treated with gastric cancer cell supernatant were substantially thickened and contained extensive fibrosis. Conclusions: These findings demonstrate that supernatants of gastric cancer cells can induce apoptosis and fibrosis in HMrSV5 human peritoneal mesothelial cells through supernatants in the early peritoneal metastasis, in a time-dependent manner, and indicate that soluble factors in the peritoneal cavity affect the morphology and function of mesothelial cells so that the resulting environment can become favorable to peritoneal metastases.
- Subjects
CANCER cells; APOPTOSIS; FIBROSIS; PERITONEAL dialysis; CANCER treatment
- Publication
BMC Gastroenterology, 2012, Vol 12, Issue 1, p34
- ISSN
1471-230X
- Publication type
Article
- DOI
10.1186/1471-230X-12-34