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- Title
A SERS/fluorescence dual-mode immuno-nanoprobe for investigating two anti-diabetic drugs on EGFR expressions.
- Authors
Cheng, Yuqi; Cong, Lili; Qu, Xiaozhang; Zhao, Junyi; Chen, Jiamin; Li, Ping; Shi, Wei; Xu, Weiqing; Xu, Shuping
- Abstract
A surface-enhanced Raman scattering (SERS)/fluorescence dual-mode nanoprobe was proposed to assess anti-diabetic drug actions from the expression level of the epidermal growth factor receptor (EGFR), which is a significant biomarker of breast cancers. The nanoprobe has a raspberry shape, prepared by coating a dye-doped silica nanosphere with a mass of SERS tags, which gives high gains in fluorescence imaging and SERS measurement. The in situ detection of EGFR on the cell membrane surfaces after drug actions was achieved by using this nanoprobe, and the detection results agree with the enzyme-linked immunosorbent assay (ELISA) kit. Our study suggests that rosiglitazone hydrochloride (RH) may be a potential drug for diabetic patients with breast cancer, while the anti-cancer effect of metformin hydrochloride (MH) is debatable since MH slightly promotes the EGFR expression of MCF-7 cells in this study. This sensing platform endows more feasibility for highly sensitive and accurate feedback of pesticide effects at the membrane protein level.
- Subjects
METFORMIN; EPIDERMAL growth factor receptors; SERS spectroscopy; FLUORESCENCE; ENZYME-linked immunosorbent assay; MEMBRANE proteins
- Publication
Microchimica Acta, 2023, Vol 190, Issue 4, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-023-05705-2