The article presents information on Pacific Biosciences (PacBio) and its single molecule, real time (SMRT) system for single-molecule sequencing. PacBio has a software team led by vice president (VP) Kevin Corcoran that the company depends on to handle the data from its single-molecule sequencer in 2010. According to team member Scott Helgesen, real-time processing of the data is the biggest challenge.