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- Title
Direct organogenesis from hypocotyl-derived explants of annatto (Bixa orellana).
- Authors
Vespasiano Borges de Paiva Neto; Tiago Ribeiro da Mota; Wagner Campos Otoni
- Abstract
The relative importance of explant, cytokinin type, carbon source and gelling agent for annatto organogenesis was studied. The best organogenic response, including adventitious shoot number and elongation, was obtained when hypocotyl segments and rooted hypocotyls were cultured onto MS medium supplemented with 4.56 μM zeatin, 87.6 mM sucrose, and 2.8 g l−1 Phytagel®. Adventitious shoots derived from hypocotyl segments were less frequent and more difficult to elongate than those derived from rooted hypocotyls. Thidiazuron (TDZ) promoted a higher organogenic response in rooted hypocotyls, resembling a rosette-like structure, but impaired shoot elongation. Histological investigation showed that zeatin-induced meristemoids originated mainly from wounding tissues, and that TDZ induced a high level of mitotic division resulting in several proliferation zones nearby the epidermis and outer cortical tissues. Rhizogenesis efficiency (rooting frequency and root number) was greater at the highest indole-3-butyric acid (IBA) concentration (5.0 μM) employed, although calli occurred at the basal end of shoots. Eighty percent of rooted plantlets survived after acclimatization. This optimized regeneration protocol may enable further development of an efficient genetic transformation protocol for this species.
- Publication
Plant Cell, Tissue & Organ Culture, 2003, Vol 75, Issue 2, p159
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1023/a:1025063906822