Two new secolignans, 3S,4S4bis4hydroxy3methoxyphenylmethyl2oxotetrahydrofuran3ylmethyl βDglucopyranoside 1 and its stereoisomer 3S,4R4bis4hydroxy3methoxyphenylmethyl2oxotetrahydrofuran3ylmethyl βDglucopyranoside 2, have been isolated from the roots of Urtica fissaE. Pritz. Their structures were determined based on spectroscopic methods HRMS, 1D and 2DNMR, HMBC, HSQC, and NOESY.