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- Title
Acamprosate inhibits Ca<sup>2+</sup> influx mediated by NMDA receptors and voltage-sensitive Ca<sup>2+</sup> channels in cultured rat mesencephalic neurones.
- Authors
Allgaier, Clemens; Franke, Heike; Sobottka, Helga; Scheibler, Peter
- Abstract
Acamprosate has recently been introduced in relapse prophylaxis in weaned alcoholics. Using fura-2 microfluorimetry, the present study investigates whether acamprosate affects N-methyl-d-aspartate (NMDA) or K+-induced changes in free intracellular Ca2+ concentration ([Ca2+]i) in rat cultured mesencephalic neurones. Both application of NMDA (plus glycine) and elevation of extracellular K+ induced rapid increases in [Ca2+]i which respectively were insensitive and sensitive to ω-conotoxin (ω-CTX) MVIIC, a blocker of voltage-dependent Ca2+ channels (VDCCs). Acamprosate (100 µM and 300 µM) significantly attenuated the response induced by NMDA as well as that induced by K+ in a concentration-dependent manner. Concurrent application of ω-CTX MVIIC and acamprosate impaired the K+-induced increase in [Ca2+]i to the same extent as ω-CTX MVIIC alone. The present data suggest that acamprosate inhibits Ca2+ influx through both NMDA receptors and VDCCs.
- Subjects
ACAMPROSATE; HORMONE receptors; BRAIN stem; CELL culture; PEOPLE with alcoholism; MESENCEPHALON; ACETIC acid
- Publication
Naunyn-Schmiedeberg's Archives of Pharmacology, 2000, Vol 362, Issue 4/5, p440
- ISSN
0028-1298
- Publication type
Article
- DOI
10.1007/s002100000285