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- Title
Detection of the cytochrome b mutation G143A in Irish Rhynchosporium commune populations using targeted 454 sequencing.
- Authors
Phelan, Sinead; Barthe, Marie-Sophie; Tobie, Camille; Kildea, Steven
- Abstract
BACKGROUND Rhynchosporium commune is a major fungal pathogen of barley crops, and the application of fungicides, such as quinone outside inhibitors ( QoIs), plays an important role in crop disease control. The genetic mechanisms linked to QoI resistance have been identified in the cytochrome b gene, with QoI resistance conferred by the G143A substitution. The objective of this study was to develop a high-throughput molecular assay to detect and identify mutations associated with QoI resistance within the Irish R. commune population. RESULTS Leaf lesions of R. commune sampled from 74 sites during 2009-2014 and isolates from 2006 and 2007 were screened for non-synonymous mutations of the cytochrome b gene using 454 targeted sequencing. The presence of the G143A substitution was confirmed in R. commune samples at one site in 2013 and at four sites in 2014; however, the frequency of the substitution in these samples was low (2-18%). The 454 sequencing results were confirmed by PCR-RFLP and Sanger sequencing. CONCLUSION The molecular assay that has been applied to this monitoring programme has shown that the application of 454 next-generation sequencing offers the potential for high throughput and accurate characterisation of non-synonymous mutations associated with fungicide resistance in a crop pathogen. © 2016 Society of Chemical Industry
- Subjects
CYTOCHROME b; RHYNCHOSPORIUM; FUNGICIDES; QUINONE; DISEASE resistance of plants
- Publication
Pest Management Science, 2017, Vol 73, Issue 6, p1154
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.4434