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- Title
Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors.
- Authors
Bernemann, Vincent; Fitschen, Jürgen; Leupold, Marco; Scheibenbogen, Karl-Heinz; Maly, Marc; Hoffmann, Marko; Wucherpfennig, Thomas; Schlüter, Michael
- Abstract
The reliable transfer of bioprocesses from single-use bioreactors (SUBs) of different scales to conventional stainless steel stirred-tank bioreactors is of steadily growing interest. In this publication, a scale-up study for SUBs with volumes of 200 L and 2000 L and the transfer to an industrial-scale conventional stainless steel stirred-tank bioreactor with a volume of 15,000 L is presented. The scale-up and transfer are based on a comparison of mixing times and the modeling of volumetric mass transfer coefficients kLa, measured in all three reactors in aqueous PBS/Kolliphor solution. The mass transfer coefficients are compared with the widely used correlation of van't Riet at constant stirrer tip speeds. It can be shown that a van't Riet correlation enables a robust and reliable prediction of mass transfer coefficients on each scale for a wide range of stirrer tip speeds and aeration rates. The process transfer from single-use bioreactors to conventional stainless steel stirred-tank bioreactors is proven to be uncritical concerning mass transfer performance. This provides higher flexibility with respect to bioreactor equipment considered for specific processes.
- Subjects
MASS transfer coefficients; STAINLESS steel; BIOREACTORS; MASS transfer
- Publication
Fluids, 2024, Vol 9, Issue 5, p115
- ISSN
2311-5521
- Publication type
Article
- DOI
10.3390/fluids9050115