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- Title
Nonequivalent nuclear location of immunoglobulin alleles in B lymphocytes.
- Authors
Skok, Jane A.; Brown, Karen E.; Azuara, Veronique; Caparros, Marie-Laure; Baxter, Jonathan; Takacs, Katalin; Dillon, Niall; Gray, David; Perry, Robert P.; Merkenschlager, Matthias; Fisher, Amanda G.
- Abstract
Individual B lymphocytes normally express immunoglobulin (Ig) proteins derived from single Ig heavy chain (H) and light chain (L) alleles. Allelic exclusion ensures monoallelic expression of Ig genes by each B cell to maintain single receptor specificity. Here we provide evidence that at later stages of B cell development, additional mechanisms may contribute to prioritizing expression of single IgH and IgL alleles. Fluorescent in situ hybridization analysis of primary splenic B cells isolated from normal and genetically manipulated mice showed that endogenous IgH, κ and λ alleles localized to different subnuclear environments after activation and had differential expression patterns. However, this differential recruitment and expression of Ig alleles was not typically seen among transformed B cell lines. These data raise the possibility that epigenetic factors help maintain the monoallelic expression of Ig.
- Subjects
IMMUNOGLOBULINS; LYMPHOCYTES; B cells
- Publication
Nature Immunology, 2001, Vol 2, Issue 9, p848
- ISSN
1529-2908
- Publication type
Article
- DOI
10.1038/ni0901-848