We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Application of Adaptive Model Predictive Control for Fed-Batch Escherichia coli BL21 (DE3) Cultivation and Biosynthesis of Recombinant Proteins.
- Authors
Dubencovs, Konstantins; Suleiko, Arturs; Sile, Elina; Petrovskis, Ivars; Akopjana, Inara; Suleiko, Anastasija; Galvanauskas, Vytautas; Tars, Kaspars; Vanags, Juris
- Abstract
A model predictive control (MPC) method was investigated as a route to optimize and control the growth of E. coli BL21 (DE3) and biosynthesis of two different recombinant proteins (nerve growth factor NGF and coat protein of bacteriophage Qβ (Qβ-CP)). To determine the target trajectory for the E. coli cultivation process and estimate the model parameters, the off-line run-to-run optimization method was used. The proven method allowed us to successfully control the growth of microbial biomass, with a deviation of 6–12% from the target trajectory. It was proven that it is possible to obtain a "Golden Batch" profile for the implementation of MPC using datasets from only four to eight fermentation runs. The method showed its robustness when the cultivation of E. coli was carried out with two different titrant supply control systems—volumetric and gravimetric. Furthermore, the MPC method exhibited high adaptability, reliability, and resistance to various types of disturbances. MPC proved to be a reliable and effective method for controlling the cultivation and recombinant protein biosynthesis of fast-growing microorganisms such as E. coli.
- Subjects
RECOMBINANT proteins; ESCHERICHIA coli; PROTEIN synthesis; PREDICTION models; NEUROTROPHIN receptors
- Publication
Fermentation (Basel), 2023, Vol 9, Issue 12, p1015
- ISSN
2311-5637
- Publication type
Article
- DOI
10.3390/fermentation9121015