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- Title
元蘑多糖分离纯化和结构表征及体外降血糖活性.
- Authors
石 越; 张玲赫; 努尔买买提; 李文文
- Abstract
Crude polysaccharide of Hohenbuehelia serotina was obtained by water extraction and alcohol precipitation, and then a polysaccharide fraction named HSP was purified from the crude polysaccharide extract by dextran gel column chromatography. The crude polysaccharide was determined for extraction rate, total sugar content and protein content. HSP was measured for its total sugar and protein contents, determined for relative molecular weight by HPLC, analyzed for monosaccharide composition by ion chromatography, and analyzed for functional groups by Fourier infrared spectroscopy. HSP was also studied for its hypoglycemic activity. The results showed that the extraction rate, total sugar content, and protein content of the crude polysaccharide were (11.83±0.08)%, (38.27±0.66)%, and (1.84±0.02)%, respectively. The total sugar and protein in HSP were (87.69±1.34)% and (0.97±0.11)%, respectively. The relative molecular weight of HSP was found to be 7.78×106. HSP is a heteropolysaccharide, comprising glucose, galactose, xylose, arabinose, galacturonic acid, fucose, rhamnose, and glucuronic acid in the molar ratio of 34.99 : 5.68 : 4.85 : 2.31 : 1.19 : 1 : 0.16 : 0.13. There are uronic acid and pyranose ring functional groups in HSP. In addition, the inhibitory activities of 3.0 mg·mL-1 HSP on α-amylase and α-glucosidase were 60.45% and 55.33%, respectively, indicating that HSP had good hypoglycemic activity in vitro.
- Subjects
MONOSACCHARIDES; GALACTURONIC acid; ION exchange chromatography; COLUMN chromatography; DEXTRAN; GLUCURONIC acid; URONIC acids; POLYSACCHARIDES
- Publication
Acta Edulis Fungi, 2023, Vol 30, Issue 4, p59
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2023.04.007