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- Title
电击法转化金针菇菌丝碎片表达报告基因 eGFP 及 GUS 的研究.
- Authors
刘建雨; 张美彦; 张丹; 徐珍; 宋春艳; 李巧珍; 尚晓冬; 谭琦; 黄志勇; 胡卫红; 张引芳; 赵静; 王瑞娟
- Abstract
Using mycelial fragments of monokaric Flammulina velutipes strain Dan3 from incomplete enzymatic digestion as the receptor, plasmid pYN6981 was transformed into the genome of F. velutipes via electroporation. The results showed that the transformed colonies grew well on the secondary screening plates, contained the hygromycin resistance gene fragment in their genomes, emitted green fluorescence under the confocal laser scanning microscopy, and displayed blue color under the GUS staining. These results suggested successful genomic insertion and expression of the plasmid. The transformation efficiency was 16 transformants per 106 mycelial fragments. The mitosis stability test showed that 80% of the transformants were genetically stable.
- Publication
Acta Edulis Fungi, 2018, Vol 25, Issue 2, p23
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2018.02.002