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- Title
Antibacterial Activity of Muscid and Myiasis Flies Larval Secretions.
- Authors
ZEREK, Aykut; YAMAN, Mehmet; GÜÇLÜ, Feyzullah
- Abstract
Maggot therapy is a method based on the use of myiasis fly larvae also called maggot which affinity necrotic tissue in wound treatment. In this study, it was aimed to determine the antibacterial activity some muscid and myiasis flies larvae secretions in vitro. In this study, Lucilia sericata, Chrysomya albiceps, Calliphora vicina and Musca domestica flies catched and placed in the cage to provide feeding and laying. After disinfected the eggs was transferred to sterile medium including sheep blood agar (5 %) and incubated overnight at 25 °C. After hatched, larvae were kept in the medium for 2-3 days and disinfected, incubated overnight at 25 °C in steril bi-distilled water (ddH2O). After incubation, the liquid which containing larval secretions, centrifuged and sterilized by filtration (0.2 μm) that bacteria could not pass through. Staphylococcus aureus, MRSA ATCC 43300, E. coli and P. aeruginosa the suspension of bacteria spreaded petri dishes including MHA sterile-medium. Secretions amount of 30 μl obtained from larvae was impregnated blank antibiotic discs and were placed in the MHA medium and then incubated overnight at 35 °C. When the results were examined, antibacterial activity were not detected against Gram-negative E. coli and P. aeruginosa bacterial strains at the larval secretions of muscid and myiasis flies, antibacterial activity was detected against Gram-positive S. aureus bacterial strain. While antibacterial activity was not detected against Gram-positive MRSA ATCC 43300 bacterial strain the larval secretion of muscid fly, antibacterial activity were detected the larval secretions of myiasis flies. In conclusion, the antibacterial activities of muscid and myiasis fly larval secretions were showed and it is suggested that studies regarding this field can be investigated by using different method and medium.
- Subjects
MYIASIS; HOUSEFLY; MAGGOT therapy; SECRETION; PSEUDOMONAS aeruginosa; TISSUE wounds; INSECT larvae
- Publication
Van Veterinary Journal, 2019, Vol 30, Issue 2, p77
- ISSN
2149-3359
- Publication type
Article